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Affinity Biosciences
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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: JNK Signaling Pathway Mediates Acetaminophen-Induced Hepatotoxicity Accompanied by Changes of Glutathione S-Transferase A1 Content and Expression
doi: 10.3389/fphar.2019.01092
Figure Lengend Snippet: Activation of JNK signaling pathway under different dosages of APAP. (A) Western blot analyses of total tissue lysate for p-JNK, JNK, p-c-Jun, c-Jun, p-c-Fos, c-Fos, and β-actin (loading control). (B – D , F , G) Change of JNK activation (fold of p-JNK/JNK), c-Jun activation (fold of p-c-Jun/c-Jun), c-Fos activation (fold of p-c-Fos/c-Fos), ASK1 activation (fold of p-ASK1/ASK1), and MKK4 activation (fold of p-MKK4/MKK4) under different dosages of APAP in liver of mice with or without SP600125. (E) Western blot analyses of total tissue lysate for p-ASK1, ASK1, p-MKK4, MKK4, and β-actin (loading control). Values represented as means ± SD, n = 3. *Represents statistical differences caused by APAP. # Represents statistical differences caused by SP600125 under 150 mg·kg −1 APAP; §Represents statistical differences caused by SP600125 under 175 mg·kg −1 APAP. 0.05 > P > 0.01 (*, # , § ). P < 0.01 (**, §§ ).
Article Snippet: Antibodies such as GSTA1 (DF6514), phospho Ser243 -c-Jun (AF3090), phospho Thr183+Tyr185 -JNK1/2/3 (AF3318), β-actin (BF0198), c-Fos (AF0132), phospho Ser362 -c-Fos (AF3053), ASK1 (AF6477),
Techniques: Activation Assay, Western Blot